Premium
Influence of electric field intensity, ionic strength, and migration distance on the mobility and diffusion in DNA surface electrophoresis
Author(s) -
Li Bingquan,
Fang Xiaohua,
Luo Haobin,
Petersen Eric,
Seo YoungSoo,
Samuilov Vladimir,
Rafailovich Miriam,
Sokolov Jonathan,
Gersappe Dilip,
Chu Benjamin
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500444
Subject(s) - electrophoresis , electric field , diffusion , analytical chemistry (journal) , ionic strength , chemistry , intensity (physics) , adsorption , ionic bonding , dna , materials science , chemical physics , chromatography , ion , optics , thermodynamics , physics , biochemistry , organic chemistry , quantum mechanics , aqueous solution
In order to increase the separation rate of surface electrophoresis while preserving the resolution for large DNA chains, e.g. , genomic DNA, the mobility and diffusion of Lambda DNA chains adsorbed on flat silicon substrate under an applied electric field, as a function of migration distance, ionic strength, and field intensity, were studied using laser fluorescence microscope. The mobility was found to follow a power law with the field intensity beyond a certain threshold. The detected DNA peak width was shown to be constant with migration distance, slightly smaller with stronger field intensity, but significantly decreased with higher ionic strength. The molecular dynamics simulation demonstrated that the peak width was strongly related with the conformation of DNA chains adsorbed onto surface. The results also implied that there was no diffusion of DNA during migration on surface. Therefore, the Nernst–Einstein relation is not valid in the surface electrophoresis and the separation rate could be improved without losing resolution by decreasing separation distance, increasing buffer concentration, and field intensity. The results indicate the fast separation of genomic DNA chains by surface electrophoresis is possible.