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Determination of different classes of amines with capillary zone electrophoresis and contactless conductivity detection
Author(s) -
Gong Xiao Yang,
Hauser Peter C.
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500423
Subject(s) - chemistry , conductivity , capillary electrophoresis , electrolyte , protonation , acetic acid , chromatography , detection limit , organic chemistry , ion , electrode
The use of contactless conductivity detection for the determination of different organic amines in CE was successfully demonstrated. Aliphatic nonUV‐absorbing species could be determined along absorbing compounds by measuring the conductivity of their protonated forms. The species tested included short‐chained aliphatic primary, secondary and tertiary amines, branched aliphatic amines, diamines, hydroxyl‐substituted amines as well as species incorporating aromatic and nonaromatic cyclic moieties. Highest sensitivity was obtained with BGE solutions containing solely acetic acid. A concentration of 0.5 M at a pH value of 2.5 was used. Detection limits were in the order of 1 μM. Complete separation of cis ‐ and trans ‐1,2‐diaminocyclohexane could be achieved by adding 18‐crown‐6 as modifier to the electrolyte solution.