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Fast immobilization of probe beads by dielectrophoresis‐controlled adhesion in a versatile microfluidic platform for affinity assay
Author(s) -
Auerswald Janko,
Widmer David,
de Rooij Nico F.,
Sigrist André,
Staubli Thomas,
Stöckli Thomas,
Knapp Helmut F.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500401
Subject(s) - dielectrophoresis , analyte , streptavidin , microfluidics , nanotechnology , biosensor , materials science , fluorescence , bead , adhesion , lab on a chip , chip , microfluidic chip , chromatography , chemistry , biotin , computer science , telecommunications , biochemistry , physics , quantum mechanics , composite material
The use of probe beads for lab‐on‐chip affinity assays is very interesting from a practical point of view. It is easier to handle and trap beads than molecules in microfluidic systems. We present a method for the immobilization of probe beads at defined areas on a chip using dielectrophoresis (DEP)‐controlled adhesion. The method is fast, i.e. , it takes between 10 and 120 s – depending on the protocol – to functionalize a chip surface at defined areas. The method is versatile, i.e. , it works for beads with different types of probe molecule coatings. The immobilization is irreversible, i.e. , the retained beads are able to withstand high flow velocities in a flow‐through device even after the DEP voltage is turned off, thus allowing the use of conventional high‐conductivity analyte buffers in the following assay procedure. We demonstrate the on‐chip immobilization of fluorescent beads coated with biotin, protein A, and goat–antimouse immunoglobulin G (IgG). The number of immobilized beads at an electrode array can be determined from their fluorescence signal. Further, we use this method to demonstrate the detection of streptavidin and mouse IgG. Finally, we demonstrate the feasibility of the parallel detection of different analyte molecules on the same chip.