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Capillary electrophoresis‐mass spectrometry of a new cross‐linker with acrylic functionality
Author(s) -
Simó Carolina,
Pérez Paloma,
Neusüß Christian,
Pelzing Matthias,
San Román Julio,
Gallardo Alberto,
Cifuentes Alejandro
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500395
Subject(s) - capillary electrophoresis , mass spectrometry , chromatography , capillary electrophoresis–mass spectrometry , chemistry , capillary action , analytical chemistry (journal) , materials science , electrospray ionization , composite material
Analytical characterization of dimethacrylate‐tyrosine‐lysine‐tyrosine (DMTLT, a new biodegradable acrylic cross‐linker synthesized at our laboratory) is carried out using CE‐MS. DMTLT is a pseudopeptide composed by tyrosine‐lysine‐tyrosine amino acids linked through urea bonds with two methacrylic groups, one at each end of the molecule, making this compound an excellent cross‐linker for polymerization reactions and for obtaining new biodegradable materials. A new CE‐MS method is developed for the characterization of DMTLT and its products of degradation after basic hydrolysis. In order to carry out an exhaustive examination of such degradation products methods based on CE coupled to IT and TOF‐MS are employed. Based on CE‐IT‐MS results and the elemental composition of the degradation products obtained by CE‐TOF‐MS, conclusions on the mechanism and kinetic of hydrolysis of DMTLT are obtained confirming both the usefulness of CE‐MS to characterize new biomaterials and the applicability of DMTLT for preparing new biodegradable polymers. These results are corroborated through the CE‐MS detection of the identified products of degradation in a dimethyl acrylamide polymer cross‐linked with DMTLT.