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Comparison of dodecoxycarbonylvaline microemulsion, solvent‐modified micellar and micellar pseudostationary phases for the chiral analysis of pharmaceutical compounds
Author(s) -
Mertzman Melissa D.,
Foley Joe P.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500351
Subject(s) - microemulsion , micelle , micellar solutions , butanol , micellar liquid chromatography , chemistry , chromatography , pulmonary surfactant , solvent , resolution (logic) , phase (matter) , n butanol , aqueous solution , organic chemistry , ethanol , biochemistry , artificial intelligence , computer science
A direct comparison of dodecoxycarbonylvaline (DDCV) microemulsion, micellar and butanol‐modified micellar aggregate systems was performed employing both 2 and 4% DDCV. With respect to either DDCV concentration, use of the micellar system provided the largest elution range, followed by the butanol‐modified micellar system and ultimately the microemulsion. Using 2% DDCV, all three aggregate analyses yielded similar values for enantioselectivity, resolution and retention factors that were slightly better using the micellar phase, but efficiencies were consistently better using either the microemulsion or butanol‐modified micellar phases. Largely, the microemulsion and butanol‐modified micellar phases behaved fairly similar, although use of the butanol‐modified micelle provided resolution and efficiency that were slightly better for all but two of the compounds. While reasonable separations were achieved using 2% DDCV, the results using 4% DDCV for the microemulsion system were far superior. Analyses using analogous micellar and butanol‐modified micellar aggregates were unstable, making them unsuitable for use at that surfactant concentration.