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An online fiber‐optic UV‐visible detector for continuous free‐flow electrophoresis
Author(s) -
Sanganza Wilson K. M.,
Ridgway Thomas H.,
Stalcup Apryll M.,
Seliskar Carl J.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500342
Subject(s) - detector , absorbance , analyte , benzoic acid , chromatography , materials science , optical fiber , flow (mathematics) , wavelength , analytical chemistry (journal) , optics , chemistry , optoelectronics , physics , organic chemistry , mechanics
A PC‐controlled, scanning online UV detector for continuous free‐flow electrophoresis (CFFE) was designed to allow for single UV wavelength monitoring across a 1‐D array of 48 longitudinal flow cells interfaced to a CFFE apparatus. In the detection scheme, the UV light is sequentially passed through each of the flow cells. The design integrates online acquisition of absorbance spectra of components separated by CFFE. Benzoic acid standard solutions were used to examine the performance of the detector. Chloroquine diphosphate and 4‐nitrophenol were used as test solutes to determine the detector's ability to distinguish analytes separated in the CFFE.