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Hydrodynamic flow and electroosmotic flow in zirconia‐packed capillaries
Author(s) -
Crosnier de Bellaistre Myriam,
Randon Jérôme,
Rocca JeanLouis
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500341
Subject(s) - cubic zirconia , phase (matter) , flow (mathematics) , electrophoresis , chemistry , analytical chemistry (journal) , packed bed , polybutadiene , electro osmosis , flow velocity , chromatography , materials science , mechanics , organic chemistry , polymer , copolymer , ceramic , physics
Fused‐silica capillaries were packed with Zirchrom®‐PBD stationary phase for application in CEC, nanoLC and pseudoelectrochromatography (PEC). Acido‐basic properties of zirconia can be used to control the EOF even if the zirconia particles were coated by polybutadiene. As for native zirconia, the EOF is pH‐dependent and the p I is close to pH 5. The mixed‐mode pressure–voltage technique induced a modulation of the mobile‐phase velocity as well as an electrophoretic migration of the solutes in order to improve the resolution of the separation. A significant increase of the flow appeared when both hydrodynamic and EOFs were in the same direction. But an important reduction of the electroosmotic velocity was observed when the hydrodynamic flow and EOF were opposed in Zirchrom‐PBD columns. This behaviour has been observed at high or low pH on several columns. Separations of neutral and charged compounds have been performed with these columns in PEC mode.