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A silica‐based monolithic column in capillary HPLC and CEC coupled with ESI‐MS or electrospray‐atmospheric‐pressure laser ionization‐MS
Author(s) -
Droste Stefan,
Schellenträger Marc,
Constapel Marc,
Gäb Siegmar,
Lorenz Matthias,
Brockmann Klaus J.,
Benter Thorsten,
Lubda Dieter,
Schmitz Oliver J.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500326
Subject(s) - chemistry , ionization , excimer laser , mass spectrometry , analytical chemistry (journal) , capillary action , atmospheric pressure , chromatography , capillary electrochromatography , analyte , laser , electrospray ionization , high performance liquid chromatography , atmospheric pressure laser ionization , capillary electrophoresis , photoionization , materials science , organic chemistry , ion , physics , oceanography , optics , composite material , geology
We describe the successful coupling of CEC and capillary HPLC with the recently developed atmospheric‐pressure laser ionization (APLI) method. APLI is suitable for selectively and sensitively ionizing nonpolar aromatic compounds at ambient pressure for subsequent mass‐selective detection. The polycyclic aromatic hydrocarbons used as analytes are first separated either by CEC on a silica‐based monolithic column or by capillary HPLC. The eluent, along with a sheath flow, is volatilized by microelectrospray and then selectively ionized by excimer laser (KrF*) radiation via two‐photon excitation. A QTOF‐MS is used as mass‐selective detector. This interface combination makes soft ionization of thermally labile nonpolar aromatic analytes possible.