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Enantiomeric separation of furan derivatives and fused polycycles by cyclodextrin‐modified micellar capillary electrophoresis
Author(s) -
Bao Ye,
Lantz Andrew W.,
Yao Tuanli,
Huang Qinhua,
Larock Richard C.,
Armstrong Daniel W.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500319
Subject(s) - capillary electrophoresis , enantiomer , cyclodextrin , furan , chemistry , chromatography , micellar electrokinetic chromatography , organic chemistry
Abstract The enantiomeric separations of highly hydrophobic furan derivatives and polycycles were performed and optimized using CD‐modified micellar CE. The most effective chiral selector for the enantiomeric separation of these analytes was hydroxypropyl‐γ‐CD. The effects of CD and SDS concentration and organic modifier were examined in order to optimize the separation conditions. The ratio of CD to surfactant concentration affected the enantiomeric separation significantly, with increases in the derivatized CD concentration generally enhancing resolution. Addition of an organic solvent modifier to the run buffer served to increase the analytes' solubility and enhance the separation efficiency. A highly acidic pH was necessary to effectively suppress the EOF when operating in the reverse polarity mode.