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Introduction of an single nucleodite polymorphism‐based “Major Y‐chromosome haplogroup typing kit” suitable for predicting the geographical origin of male lineages
Author(s) -
Brión María,
Sanchez Juan J.,
Balogh Kinga,
Thacker Catherine,
BlancoVerea Alejandro,
Børsting Claus,
StradmannBellinghausen Beate,
Bogus Magdalena,
SyndercombeCourt Denise,
Schneider Peter M.,
Carracedo Angel,
Morling Niels
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500293
Subject(s) - haplogroup , genetics , single nucleotide polymorphism , biology , genotyping , typing , y chromosome , snp genotyping , population , haplotype , allele , evolutionary biology , genotype , gene , medicine , environmental health
The European Consortium “High‐throughput analysis of single nucleotide polymorphisms for the forensic identification of persons – SNPforID”, has performed a selection of candidate Y‐chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y‐Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A “Major Y‐chromosome haplogroup typing kit” has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y‐chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.