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Chiral separations on multichannel microfluidic chips
Author(s) -
Gao Yan,
Shen Zheng,
Wang Hui,
Dai Zhongpeng,
Lin Bingcheng
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500283
Subject(s) - enantiomer , microfluidics , chip , resolution (logic) , microfluidic chip , chromatography , chemistry , beta (programming language) , combinatorial chemistry , detector , nanotechnology , materials science , stereochemistry , computer science , telecommunications , artificial intelligence , programming language
Abstract Chiral separations of FITC‐labeled basic drugs on multichannel microfluidic chips with LIF detector were investigated. A preliminary screening procedure for seven neutral CDs was performed under optimized conditions for chiral separations of three FITC‐labeled drugs (baclofen, norfenefrine, and tocainide) on a mono‐channel microfluidic chip. According to the results of screening, FITC‐baclofen and FITC‐norfenefrine as well as two chiral selectors including γ‐CD and dimethyl‐β‐CD (DM‐β‐CD) were selected as models to perform chiral separations on a two‐channel chip. FITC‐baclofen enantiomers were separated completely by γ‐CD in one channel, while resolution of FITC‐norfenefrine enantiomers was achieved by DM‐β‐CD in the other channel in the same run. Furthermore, the feasibility of using one chiral selector to separate multiple chiral samples was studied on a four‐channel chip. These results show that multichannel chip has a potential for chiral high‐throughput screening.