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Mutation scanning‐coupled analysis of haplotypic variability in mitochondrial DNA regions reveals low gene flow between human and porcine Ascaris in endemic regions of China
Author(s) -
Peng Weidong,
Yuan Keng,
Hu Min,
Zhou Xianmin,
Gasser Robin B.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500276
Subject(s) - ascaris , mitochondrial dna , biology , haplotype , gene flow , locus (genetics) , genetics , gene , genetic variation , genotype , zoology , helminths
Haplotypic variation within and among the Ascaris populations representing six provinces in China was investigated. Mitochondrial DNA regions in the cytochrome c oxidase subunit 1 ( cox 1) and NADH dehydrogenase subunit 1 ( nad 1) genes were amplified by PCR from total genomic DNA samples ( n > 720) from Ascaris individuals from humans and pigs, and subjected to mutation scanning and subsequent selective sequencing. For the cox 1, ten different electrophoretic profiles were recorded for human Ascaris , and the same number for pig Ascaris , one of them being common to both host species. For the nad 1, 11 different profiles were detected for human Ascaris , and 15 for pig Ascaris . Having defined all haplotypes (20 for p cox 1 and 26 for p nad 1) by sequencing, their frequencies were estimated in each of the two host species and each of the six provinces. For each mitochondrial region, the frequency of the different haplotypes varied considerably, depending on host species and geographical origin. Analysis of the sequence data (representing all haplotypes for each mitochondrial locus) by F ‐statistics indicated restricted gene flow between human Ascaris and pig Ascaris , and supported the conclusions from previous molecular epidemiological investigations that pigs are not a significant source of Ascaris infection in humans in endemic regions.