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Electrophoresis in capillary cells with detection gap
Author(s) -
Gavrilov Dmitri N.,
Kosobokova Olga,
Khozikov Vyacheslav,
Stepukhovitch Andrey,
Gorfinkel Vera
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500237
Subject(s) - capillary electrophoresis , electrophoresis , electric field , capillary action , materials science , optoelectronics , computer science , chromatography , chemistry , physics , quantum mechanics , composite material
Abstract A novel design of the detection zone in multicapillary arrays used for electrophoretic separation is presented. The use of a detection gap (DG), in which the reflective surfaces separating the channels of the array are eliminated, is proposed to improve the illumination and detection of the separated DNA fragments. The electric field compression in the DG is achieved by optimization of the gap geometry. The results of the computer simulation and experiment demonstrate no substantial band‐broadening in the DG. We believe that the proposed method will be useful for application in the microfabricated devices.