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Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier
Author(s) -
Yu Lijun,
Xu Yan,
Feng Huatao,
Li Sam Fong Yau
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500233
Subject(s) - micellar electrokinetic chromatography , pyrrolizidine , chromatography , chemistry , calibration curve , detection limit , stereochemistry
A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for the separation and determination of four toxic pyrrolizidine alkaloids (PAs) (senkirkine, senecionine, retrorsine, and seneciphylline) in two traditional Chinese herbal medicines ( Qian liguang and Kuan donghua ). Separation was performed in the running buffer consisting of 20 m M borate, 30 m M SDS, and 20% methanol at pH 9.1. With the optimized separation conditions, four PAs were separated in 17 min by a single run. The calibration curves showed good linearity with correlation efficiencies ( R 2 ) between 0.9940 and 0.9988. RSDs in migration time and peak area were 0.31, 0.40, 0.39, 0.48% and 3.28, 3.48, 4.16, 3.42% for senkirkine, senecionine, retrorsine, and seneciphylline, respectively. Limits of detection (S/N = 3) varied from 1.19 to 2.70 μg/mL. The proposed method was applied to determine the PAs extracted from Chinese herbal medicines ( Qian liguang and Kuan donghua ). PA of senkirkine in Kuan donghua was detected and the amount was found to be 79.1 μg/g. The results obtained indicate that the proposed MEKC method could potentially become an effective alternative tool for qualification control and quantitative analysis of herbal medicines in pharmaceutical industry.