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Fast and simultaneous detection of prominent natural antioxidants using analytical microsystems for capillary electrophoresis with a glassy carbon electrode: A new gateway to food environments
Author(s) -
Blasco Antonio Javier,
Barrigas Inés,
González María Cristina,
Escarpa Alberto
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500211
Subject(s) - capillary electrophoresis , microsystem , glassy carbon , carbon fibers , electrode , chromatography , electrophoresis , chemistry , gateway (web page) , analytical chemistry (journal) , materials science , nanotechnology , computer science , electrochemistry , cyclic voltammetry , composite material , composite number , world wide web
This paper examines for the first time the analytical possibilities of fast and simultaneous detection of prominent natural antioxidants including examples of flavonoids and vitamins using a CE microchip with electrochemical detection (ED). Unpinched injection conditions, zone electrophoretic separation and amperometric detection were carefully assayed and optimised. Analysis involved the zone electrophoretic separation of arbutin, (+)‐catechin and ascorbic acid in less than 4 min using a borate buffer (pH 9.0, 50 mM), employing 2 kV as the separation voltage and +1.0 V as the detection potential. In addition, the separation of different ‘couples’ of natural antioxidants of food significance including (+)‐catechin and ascorbic acid, (+)‐catechin and rutin, as well as arbutin and phlorizdin is proposed. To demonstrate the potential and future role of CE microsystems, analytical possibilities and a new route in the raw sample analysis are presented. The preliminary results obtained allow the proposal of CE‐ED microchips as a real gateway to microanalysis in foods.