Premium
Hydrolytically stable, diaminocarboxylic acid‐based membranes buffering in the pH range from 6 to 8.5 for isoelectric trapping separations
Author(s) -
Fleisher Helen C.,
Vigh Gyula
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500198
Subject(s) - chemistry , membrane , chromatography , isoelectric focusing , formic acid , isoelectric point , substrate (aquarium) , diglycidyl ether , histidine , vinyl alcohol , amino acid , organic chemistry , biochemistry , enzyme , bisphenol a , oceanography , epoxy , geology , polymer
Diaminocarboxylic acid carrier ampholytes, such as L ‐histidine, 2,3‐diaminopropionic acid, L ‐ornithine, and L ‐lysine, were reacted with glycerol‐1,3‐diglycidyl ether (GDGE) and poly(vinyl alcohol) (PVA) in the presence of sodium hydroxide to produce hydrolytically and mechanically stable hydrogels, supported on a PVA substrate, for use as buffering membranes in isoelectric trapping (IET) separations. The pH values of the DACAPVA membranes were determined with the help of small‐molecule p I markers and proteins and were found to be in the 6 < pH < 8.5 range. The membranes were successfully used to isoelectrically trap small ampholytes, desalt ampholyte solutions in IET mode, and effect the binary separation of chicken egg white proteins.