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Separation of porphyrin‐based photosensitizer isomers by laser‐induced fluorescence capillary electrophoresis
Author(s) -
Peng Xuejun,
Sternberg Ethan,
Dolphin David
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500183
Subject(s) - photosensitizer , chemistry , capillary electrophoresis , enantiomer , porphyrin , structural isomer , fluorescence , derivative (finance) , chromatography , analytical chemistry (journal) , photochemistry , organic chemistry , physics , quantum mechanics , financial economics , economics
Methods for the separation of photosensitizer isomers, such as benzoporphyrin derivative monoacid, benzoporphyrin ethyl monoacid, 2‐[1‐hexyloxyethyl]‐2‐devinylpyropheophorbide‐a, diethyleneglycol diester benzoporphyrin derivative, tin ethyl etiopurpurin, and phthalocyanine tetrasulfonate, have been systematically developed by CE. Detection was accomplished by UV absorption at 214 nm or by LIF with excitation at 442/488 nm and emission at 690 nm. The effects of three major experimental parameters of buffer types, organic solvents, and surfactant additives are described. The optimized separation conditions were determined so as to provide satisfactory separation efficiency and analysis time. The methods are shown to be suitable for the separation and determination of porphyrin and phthalocyanines regioisomers, diastereoisomers, and enantiomers.