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Utility of lab‐on‐a‐chip technology for high‐throughput nucleic acid and protein analysis
Author(s) -
Hawtin Paul,
Hardern Ian,
Wittig Rainer,
Mollenhauer Jan,
Poustka AnneMarie,
Salowsky Ruediger,
Wulff Tanja,
Rizzo Christopher,
Wilson Bill
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500166
Subject(s) - multiplex , throughput , nucleic acid , computer science , dna microarray , reproducibility , chromatography , computational biology , chemistry , biology , bioinformatics , gene , gene expression , biochemistry , telecommunications , wireless
On‐chip electrophoresis can provide size separations of nucleic acids and proteins similar to more traditional slab gel electrophoresis. Lab‐on‐a‐chip (LoaC) systems utilize on‐chip electrophoresis in conjunction with sizing calibration, sensitive detection schemes, and sophisticated data analysis to achieve rapid analysis times (< 120 s). This work describes the utility of LoaC systems to enable and augment systems biology investigations. RNA quality, as assessed by an RNA integrity number score, is compared to existing quality control (QC) measurements. High‐throughput DNA analysis of multiplex PCR samples is used to stratify gene sets for disease discovery. Finally, the applicability of a high‐throughput LoaC system for assessing protein purification is demonstrated. The improvements in workflow processes, speed of analysis, data accuracy and reproducibility, and automated data analysis are illustrated.