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Poly(methylmethacrylate) and Topas capillary electrophoresis microchip performance with electrochemical detection
Author(s) -
CastañoÁlvarez Mario,
FernándezAbedul María T.,
CostaGarcía Agustín
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500148
Subject(s) - capillary electrophoresis , capillary action , chromatography , electrophoresis , materials science , electrochemistry , analytical chemistry (journal) , chemistry , composite material , electrode
A capillary electrophoresis (CE) microchip made of a new and promising polymeric material: Topas (thermoplastic olefin polymer of amorphous structure), a cyclic olefin copolymer with high chemical resistance, has been tested for the first time with analytical purposes, employing an electrochemical detection. A simple end‐channel platinum amperometric detector has been designed, checked, and optimized in a poly‐(methylmethacrylate) (PMMA) CE microchip. The end‐channel design is based on a platinum wire manually aligned at the exit of the separation channel. This is a simple and durable detection in which the working electrode is not pretreated. H 2 O 2 was employed as model analyte to study the performance of the PMMA microchip and the detector. Factors influencing migration and detection processes were examined and optimized. Separation of H 2 O 2 and L ‐ascorbic acid (AsA) was developed in order to evaluate the efficiency of microchips using different buffer systems. This detection has been checked for the first time with a microchip made of Topas, obtaining a good linear relationship for mixtures of H 2 O 2 and AsA in different buffers.