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Effect of polymer matrix and glycerol on rapid single‐strand conformation polymorphism analysis by capillary and microchip electrophoresis for detection of mutations in K‐ras gene
Author(s) -
Endo Yuki,
Zhang Lihua,
Katashima Rumi,
Itakura Mitsuo,
Doherty Erin A. S.,
Barron Annelise E.,
Baba Yoshinobu
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500142
Subject(s) - capillary electrophoresis , single strand conformation polymorphism , mutant , microbiology and biotechnology , gene , polymer , electrophoresis , polymorphism (computer science) , gel electrophoresis , mutation , chemistry , biology , genetics , genotype , organic chemistry
We present the rapid single‐strand conformation polymorphism (SSCP) analysis by capillary and microchip electrophoresis to detect the mutations in K‐ras gene. Parameters that might affect the analysis of mutation in K‐ras gene, such as the polymer and the additive in the sieving matrix, have been studied systematically. Under the optimal conditions, the analysis of seven mutants of K‐ras gene could be finished within 10 min by capillary electrophoresis (CE). Furthermore, with the wild‐type gene as the inner standard, the analysis accuracy of mutations could be improved. In addition, by studying the properties of polymer solutions, the matrix suitable for microchip electrophoresis was found, and the detection of mutations in K‐ras gene could be further shortened to 1 min.