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Chiral separation of halogenated amino acids by ligand‐exchange capillary electrophoresis
Author(s) -
Koidl Julia,
Hödl Heike,
Schmid Martin G.,
Pantcheva Svetlana,
Pajpanova Tamara,
Gübitz Gerald
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500130
Subject(s) - amino acid , hydroxyproline , chemistry , histidine , ligand (biochemistry) , resolution (logic) , capillary electrophoresis , chiral ligand , chromatography , alkyl , electrophoresis , organic chemistry , catalysis , enantioselective synthesis , biochemistry , receptor , artificial intelligence , computer science
The chiral separation of halogenated amino acids by ligand‐exchange CE is described. Halogenated amino acids attracted increasing interest in recent years because of their physiological activities. Different chiral selectors, as there are L ‐4‐hydroxyproline, L ‐histidine, and N ‐alkyl derivatives of L ‐4‐hydroxyproline in form of their copper(II) complexes, are compared for their chiral recognition ability for halogenated amino acids. The influence of various parameters, such as selector concentration, pH, organic modifier, and field strength, on the resolution was investigated. All halogenated amino acids investigated were baseline‐separated under optimized conditions.