Premium
Analysis of amino acids and proteins using a poly(methyl methacrylate) microfluidic system
Author(s) -
Kato Masaru,
Gyoten Yukari,
SakaiKato Kumiko,
Nakajima Tohru,
Toyo'oka Toshimasa
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500124
Subject(s) - analyte , cationic polymerization , methyl methacrylate , chemistry , adsorption , fluorescence , amino acid , chromatography , polymer , methacrylate , derivative (finance) , polymer chemistry , organic chemistry , copolymer , biochemistry , physics , quantum mechanics , economics , financial economics
Plastic microchips are very promising analytical devices for the high‐speed analysis of biological compounds. However, due to its hydrophobicity, their surface strongly interacts with nonpolar analytes or species containing hydrophobic domains, resulting in a significant uncontrolled adsorption on the channel walls. This paper describes the migration of fluorescence‐labeled amino acids and proteins using the poly(methyl methacrylate) microchip. A cationic starch derivative significantly decreases the adsorption of analytes on the channel walls. The migration time of the analytes was related to their molecular weight and net charge or p I of the analytes. FITC‐BSA migrated within 2 min, and the theoretical plate number of the peak reached 480 000 plates/m. Furthermore, proteins with a wide range of p I values and molecular weights migrated within 1 min using the microchip.