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Enantioseparation of phenothiazines in cyclodextrin‐modified capillary zone electrophoresis using sulfated cyclodextrins as chiral selectors
Author(s) -
Lin ChingErh,
Liao WeiSsu,
Cheng HsuTun,
Kuo ChiaMing,
Liu YuChih
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500087
Subject(s) - capillary electrophoresis , cyclodextrin , sulfation , chromatography , chemistry , capillary electrochromatography , capillary action , electrophoresis , materials science , biochemistry , composite material
In this study, enantioseparations of five phenothiazines, including promethazine, ethopropazine, trimeprazine, methotrimeprazine, and thioridazine, in CD‐modified CZE using dual CD systems consisting of randomly sulfate‐substituted CD (MI‐S‐β‐CD) and a neutral CD as chiral selectors in a citrate buffer (100 m M ) at pH 3.0 were investigated. The results indicate that MI‐S‐β‐CD is an excellent chiral selector for enantioseparation of ethopropazine. The enantiomers of promethazine can also be baseline‐resolved with MI‐S‐β‐CD at concentrations in the range of 0.5–1.0% w/v. On the other hand, thioridazine and trimeprazine interact strongly with neutral CDs. As a result, the enantioselectivity of these two phenothiazines is remarkably and synergistically enhanced with increasing the concentration of neutral CDs in the presence of MI‐S‐β‐CD and simultaneous enantioseparations of these phenothiazines, except for methotrimeprazine, could favorably be achieved with the use of dual CD systems. Moreover, by varying the concentration of β‐CD or γ‐CD at a fixed concentration of MI‐S‐β‐CD (0.75% w/v) reversal of the enantiomer migration order of promethazine occurred. This may be attributable to the opposite effects of charged and neutral CDs on the mobility of the enantiomers of promethazine.