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Strategy for the chiral separation of non‐acidic pharmaceuticals using capillary electrochromatography
Author(s) -
Mangelings Debby,
Discry Jérôme,
Maftouh Mohamed,
Massart D. Luc,
Vander Heyden Yvan
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500079
Subject(s) - capillary electrochromatography , chemistry , bifunctional , chromatography , chiral stationary phase , phosphate buffered saline , electrochromatography , phosphate , combinatorial chemistry , baseline (sea) , stationary phase , high performance liquid chromatography , catalysis , organic chemistry , oceanography , geology
In completion of an earlier defined generic chiral screening approach, a generic separation strategy for basic, bifunctional, and neutral compounds was proposed and evaluated. This strategy adds to a previously defined strategy for acidic compounds. The screening experiment of the actual strategy used a mobile phase of 5 m M phosphate buffer pH 11.5/ACN (30/70 v/v), a temperature of 25°C, and a voltage of 15 kV. The selected chiral stationary phases were Chiralpak AD‐RH, Chiralcel OD‐RH, Chiralcel OJ‐RH, and Chiralpak AS‐RH, all based on polysaccharide selectors. It was seen that 31 out of 48 test compounds were partially or baseline‐resolved under screening conditions. After execution of the optimization steps of the strategy, this number increased to 41, with a total of 21 baseline‐separated compounds. Combined with the results obtained from the acidic test set examined in the earlier defined strategy, of all tested compounds 82.5% showed enantioselectivity and 49.2% could be baseline‐separated.