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Separation and determination of four active anthraquinones in Chinese herbal preparations by flow injection‐capillary electrophoresis
Author(s) -
Liu Lihong,
Fan Liuyin,
Chen Hongli,
Chen Xingguo,
Hu Zhide
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500069
Subject(s) - anthraquinones , capillary electrophoresis , chromatography , chemistry , capillary action , electrophoresis , materials science , biology , botany , composite material
A simple, rapid, and accurate method for the separation and determination of physcion, chrysophanol, aloe‐emodin, and emodin in Rhubarb, Juemingzi, and Chinese herbal preparations was developed by combination of flow injection‐capillary zone electrophoresis for the first time. The analysis was carried out using an unmodified fused‐silica capillary (75 mm × 50 μm ID × 375 μm OD, effective separation length of 48 mm) and direct ultraviolet detection at 254 nm. By a series of optimization, the sample solvent consisted of NaOH (100 mmol/L) and ACN (1:1 v/v), and a running buffer composed of 15 mmol/L sodium borate – 12.5 mmol/L sodium dihydrogen phosphate – 42% v/v ACN (pH 10.1) was applied for the separation of the four anthraquinones. The separation was rapid and highly reproducible, with complete resolution of all four compounds within 6 min. The sample throughput rate could reach up to 12 per h. The repeatability (defined as relative standard deviation) was 4.45, 4.44, 4.34, 0.61% with peak height evaluation and 1.62, 0.89, 2.49, 2.19% with peak area evaluation for physcion, chrysophanol, aloe‐emodin, and emodin, respectively.