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Comparison of methanol and acetonitrile as solvents for the separation of sertindole and its major metabolites by capillary zone electrophoresis
Author(s) -
Subirats Xavier,
Reinstadler Sigrun,
Porras Simo P.,
Raggi Maria Augusta,
Kenndler Ernst
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500056
Subject(s) - capillary electrophoresis , chromatography , acetonitrile , chemistry , methanol , sertindole , capillary electrochromatography , organic chemistry , biology , neuroscience , dopamine , haloperidol
Sertindole (1‐[2‐[4‐[5‐chloro‐1‐(4‐fluorophenyl)‐1 H ‐indol‐3‐yl]‐1‐piperidinyl]ethyl]‐2‐imidazolidinone), an atypical antipsychotic drug, was separated by capillary electrophoresis from its two main metabolites norsertindole and dehydrosertindole. The low solubility of the analytes in water (octanol‐water partition coefficient is about 10 5 ) is overcome by the use of methanol (MeOH) and acetonitrile (ACN) as solvents for the background electrolyte (BGE). Mobilities were measured in BGEs with defined pH in a broad range. It was found that in MeOH the mobility of the analytes is mainly governed by acid–base equilibria, whereas in ACN other reactions like ion pairing and homoconjugation play a pronounced role and lead to a complex pattern of the mobility as function of the pH. However, separation can be obtained in less than 10 min in both solvent systems.