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Chiral separation in capillary electrophoresis using dual neutral cyclodextrins: Theoretical models of electrophoretic mobility difference and separation selectivity
Author(s) -
Nhujak Thumnoon,
Sastravaha Chatvadee,
Palanuvej Chanida,
Petsom Amorn
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500028
Subject(s) - enantiomer , capillary electrophoresis , chemistry , selectivity , electrophoresis , cyclodextrin , analyte , chromatography , beta (programming language) , analytical chemistry (journal) , stereochemistry , organic chemistry , computer science , programming language , catalysis
Simple equations and theoretical models, related to enantioselectivity ( κ ) and C , have been developed for prediction of electrophoretic mobility difference (Δ μ ) and separation selectivity ( α ) for enantiomers in CE using dual CDs, where α and κ are defined as the ratio of μ and the ratio of binding constant ( K ) for enantiomers to each CD, respectively, C the CD concentration, and the average K for enantiomers and each CD. Experiments were carried out using dual CDs as β‐CD and dimethyl‐β‐cyclodextrin (DM‐β‐CD) and test analytes as five pairs of amphetamine drug enantiomers. A change in observed Δ μ and α of enantiomers in dual CDs was found to be in excellent agreement with the theoretical models. For example, in comparison with single CD1, dual CDs can enhance Δ μ and α up to the maximum value when enantiomers migrate with the same order in CD1 and CD2, and have the value of ρ > 1.0, where ρ is the enantioselectivity ratio for CD2 to CD1, while worse Δ μ and α are obtained for enantiomers with ρ < 1.0.