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System zones in capillary zone electrophoresis: Moving boundaries caused by freely migrating hydroxide ions
Author(s) -
Beckers Jozef L.,
Urbánek Marek,
Boček Petr
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200500006
Subject(s) - hydroxide , capillary electrophoresis , capillary action , electromigration , chemistry , ion , electrolyte , dispersion (optics) , analytical chemistry (journal) , electrophoresis , chromatography , inorganic chemistry , materials science , physics , optics , composite material , organic chemistry , electrode
We present theoretical and experimental data indicating that anionic system zones (SZs), due to free migrating hydroxide anions, can be expected in background electrolytes (BGEs) with a low buffer capacity. In the system containing completely unbuffered BGEs the hydroxide ions derived from the sample start to migrate freely through the capillary tube with the mobility of single hydroxide ions and cause stepwise disturbances in the baseline of the detector trace. Remarkably, this type of SZs do not appear to contribute significantly to the electromigration dispersion (EMD) of the zones of the analytes.