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Nonradioactive telomerase activity assay by microchip electrophoresis: Privileges to the classical gel electrophoresis assay
Author(s) -
Zhelev Zhivko,
Bakalova Rumiana,
Ewis Ashraf,
Ohba Hideki,
Ishikawa Mitsuru,
Baba Yoshinobu
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410435
Subject(s) - telomerase , electrophoresis , gel electrophoresis , microbiology and biotechnology , polymerase chain reaction , chromatography , chemistry , biology , biochemistry , gene
The present study accents on the privileges of microchip‐based electrophoresis to the conventional gel electrophoresis in separation of telomerase repeat amplification protocol/polymerase chain reaction (PCR) ladder products obtained in telomerase‐catalyzed reaction in cancer cells. We try to clarify the interpretation of the results obtained by both electrophoretic procedures and to avoid misinterpretation as a result of PCR‐dependent artefacts.