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Nanoparticle‐filled capillary electrophoresis for the separation of long DNA molecules in the presence of hydrodynamic and electrokinetic forces
Author(s) -
Tseng WeiLung,
Huang MingFeng,
Huang YuFen,
Chang HuanTsung
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410433
Subject(s) - electrokinetic phenomena , capillary electrophoresis , electrophoresis , nanoparticle , gel electrophoresis of nucleic acids , dna , electric field , capillary action , analytical chemistry (journal) , polymer , molecule , chemistry , chromatography , quenching (fluorescence) , materials science , fluorescence , chemical physics , nanotechnology , composite material , physics , biochemistry , organic chemistry , quantum mechanics
We report the analysis of long DNA molecules by nanoparticle‐filled capillary electrophoresis (NFCE) under the influences of hydrodynamic and electrokinetic forces. The gold nanoparticle (GNP)/polymer composites (GNPPs) prepared from GNPs and poly(ethylene oxide) were filled in a capillary to act as separation matrices for DNA separation. The separations of λ‐DNA (0.12–23.1 kbp) and high‐molecular‐weight DNA markers (8.27–48.5 kbp) by NFCE, under an electric field of —140 V/cm and a hydrodynamic flow velocity of 554 μm/s, were accomplished within 5 min. To further investigate the separation mechanism, the migration of λ‐DNA was monitored in real time using a charge‐coupled device (CCD) imaging system. The GNPPs provide greater retardation than do conventional polymer media when they are encountered during the electrophoretic process. The presence of interactions between the GNPPs and the DNA molecules is further supported by the fluorescence quenching of prelabeled λ‐DNA, which occurs through an energy transfer mechanism. Based on the results presented in this study, we suggest that the electric field, hydrodynamic flow, and GNPP concentration are the three main determinants of DNA separation in NFCE.