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Comparison of the use of aqueous and nonaqueous buffers in association with cyclodextrin for the chiral separation of 3,4‐methylenedioxymethamphetamine and related compounds
Author(s) -
Huang YuSan,
Tsai ChungChen,
Liu JuTsung,
Lin ChengHuang
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410374
Subject(s) - chemistry , aqueous solution , chromatography , cyclodextrin , capillary electrophoresis , metabolite , mdma , organic chemistry , pharmacology , biochemistry , medicine
A comparison of the use of aqueous and nonaqueous buffers in association with β‐CD for the chiral separation of ( R )‐ and ( S )‐3,4‐methylenedioxymethamphetamine and related compounds is described. The ( R )‐ and ( S )‐isomers of 3,4‐methylenedioxymethamphetamine (MDMA) and its major metabolite 3,4‐methylenedioxyamphetamine (MDA) were prepared. Under aqueous and nonaqueous buffer conditions and based on the CZE and MEKC modes, the order of migration of ( R )‐MDA, ( S )‐MDA, ( R )‐MDMA, and the ( S )‐MDMA enantioisomers were determined. Several electrophoretic parameters, including the concentration of β‐CD (aqueous, 25–60 m M ; nonaqueous, 20–150 m M ) used in the electrophoretic separation and the amount of organic solvents required for the separation, were optimized.