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Conductivity detection in capillary zone electrophoresis: Inspection by PeakMaster
Author(s) -
Jaroš Michal,
Soga Tomoyoshi,
van de Goor Tom,
Gaš Bohuslav
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410366
Subject(s) - electromigration , conductivity , capillary electrophoresis , analyte , electrolyte , analytical chemistry (journal) , dispersion (optics) , electrophoresis , chemistry , chromatography , signal (programming language) , capillary action , materials science , electrode , optics , computer science , physics , composite material , programming language
A simple rule stating that the signal in conductivity detection in capillary zone electrophoresis is proportional to the difference between the analyte mobility and mobility of the background electrolyte (BGE) co‐ion is valid only for systems with fully ionized electrolytes. In zone electrophoresis systems with weak electrolytes both conductivity signal and electromigration dispersion of analyte peaks depend on the conductivity and pH effects. This allows optimization of the composition of BGEs to give a good conductivity signal of analytes while still keeping electromigration dispersion near zero, regardless of the injected amount of sample. The demands to achieve minimum electromigration dispersion and high sensitivity in conductivity detection can be accomplished at the same time. PeakMaster software is used for inspection of BGEs commonly used for separation of sugars (carbohydrates, saccharides) at highly alkaline pH. It is shown that the terms direct and indirect conductivity detection are misleading and should not be used.