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High‐speed separation of proteins by microchip electrophoresis using a polyethylene glycol‐coated plastic chip with a sodium dodecyl sulfate‐linear polyacrylamide solution
Author(s) -
Nagata Hideya,
Tabuchi Mari,
Hirano Ken,
Baba Yoshinobu
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410337
Subject(s) - sodium dodecyl sulfate , polyethylene glycol , chromatography , electrokinetic phenomena , peg ratio , electrophoresis , polyacrylamide , chemistry , polymer , gel electrophoresis , sodium , materials science , polymer chemistry , nanotechnology , organic chemistry , biochemistry , finance , economics
In this paper, we describe a method for size‐based electrophoretic separation of sodium dodecyl sulfate (SDS)–protein complexes on a polymethyl methacrylate (PMMA) microchip, using a separation buffer solution containing SDS and linear polyacrylamide as a sieving matrix. We developed optimum conditions under which protein separations can be performed, using polyethylene glycol (PEG)‐coated polymer microchips and electrokinetic sample injection. We studied the performance of protein separations on the PEG‐coated PMMA microchip. The electrophoretic separation of proteins (21.5–116.0 kDa) was completed with separation lengths of 3 mm, achieved within 8 s on the PEG‐coated microchip. This high‐speed method may be applied to protein separations over a large range of molecular weight, making the PEG‐coated microchip approach applicable to high‐speed proteome analysis systems.