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Tris(2,2'‐bipyridyl)ruthenium(II)‐zirconia‐Nafion composite films applied as solid‐state electrochemiluminescence detector for capillary electrophoresis
Author(s) -
Ding ShouNian,
Xu JingJuan,
Chen HongYuan
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410324
Subject(s) - nafion , capillary electrophoresis , ruthenium , electrochemiluminescence , cubic zirconia , analytical chemistry (journal) , chemistry , materials science , nuclear chemistry , electrode , inorganic chemistry , detection limit , electrochemistry , chromatography , organic chemistry , catalysis , ceramic
The major goal of this work was to develop a new solid‐state electrochemiluminescence (ECL) detector suitable for capillary electrophoresis (CE). The detector was fabricated by coating a sol‐gel derived zirconia (ZrO 2 )‐Nafion composite film on a graphite electrode, then the zirconia‐Nafion modified electrode was immersed in tris(2,2'‐bipyridyl)ruthenium(II) (Ru(bpy) 3 2+ ) solution to immobilize this active chemiluminescence reagent. The voltammetric and ECL behaviors of the detector were investigated and optimized in tripropylamine solution. The ratio of 53% for zirconia in the zirconia‐Nafion composite provided the highest luminescence intensity of immobilized Ru(bpy) 3 2+ . The ECL can maintain its stability very well in the phosphate solution in the period of 5–90 h when the solid‐state ECL detector was immersed in the solution all the time. The optimum distance of capillary outlet to the solid‐state ECL detector has been found to be ca. 50–80 µm for a 75 µm capillary. The effects of ionic strength and pH of ECL solution on peak height were investigated. The CE with solid‐state ECL detector system was successfully used to detect tripropylamine, lidocaine, and proline. The detection limits (S/N = 3) were 5 × 10 −9 mol·L −1 for tripropylamine, 1 × 10 −8 mol·L −1 for lidocaine and 5 × 10 −6 mol·L −1 for proline, and the linear ranges were from 1.0 × 10 −8 to 1.0 × 10 −5 mol·L −1 for tripropylamine, 5.0 × 10 −7 mol·L −1 to 1.0 × 10 −5 mol·L −1 for lidocaine and 1.0 × 10 −5 to 1.0 × 10 −3 mol·L −1 for proline, respectively.