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Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts
Author(s) -
Unger Matthias,
Laug Stefanie,
Holzgrabe Ulrike
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410322
Subject(s) - capillary electrophoresis , chromatography , berberine , detection limit , chemistry , ammonium acetate , acetic acid , traditional medicine , high performance liquid chromatography , biochemistry , medicine
The root extracts of goldenseal ( Hydrastis canadensis L.) are popular phytomedicines for the treatment of gastrointestinal disorders and upper respiratory tract infections. Here we describe a simple and fast capillary zone electrophoresis (CZE) method with ultraviolet detection at 225 nm for the quantification of the major goldenseal constituents, berberine and hydrastine, in herbal remedies containing goldenseal root extracts. Tritoqualine, an antihistaminic drug with a hydrastine‐like phthalidisoquinoline structure, was applied as an internal standard. The running buffer was a 1:5 mixture of 500 m M ammonium acetate (adjusted to pH 3.4 with acetic acid) and methanol. Our newly developed CZE method was validated regarding limit of detection (LOD), limit of quantification, linearity, accuracy and precision. For both berberine and hydrastine, the LOD was 1.0 µg/mL and the linearity was obtained between 2.5 and 500 µg/mL. Using our newly developed method, both the alkaloids could be analysed in herbal remedies containing goldenseal root extracts within 8 min.