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Field gradient electrophoresis
Author(s) -
Warnick Karl F.,
Francom Scott J.,
Humble Paul H.,
Kelly Ryan T.,
Woolley Adam T.,
Lee Milton L.,
Tolley H. Dennis
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410319
Subject(s) - electrophoresis , electric field , electric field gradient , capillary electrophoresis , taylor dispersion , analyte , potential gradient , field (mathematics) , isoelectric focusing , isotachophoresis , chemistry , analytical chemistry (journal) , chromatography , physics , mathematics , electrolyte , diffusion , biochemistry , electrode , quantum mechanics , pure mathematics , thermodynamics , enzyme
The class of equilibrium gradient methods utilizes the opposition of two forces, at least one of which changes in magnitude with position, to separate and concentrate analytes. The drawback of many methods of this type is that the production of two opposing forces requires in comparison to standard methods, such as capillary electrophoresis, a relatively complex apparatus. In addition, for techniques such as electric field gradient focusing, hydrodynamic flow leads to Taylor dispersion, which limits the attainable concentration factor. We propose a new method, gradient field electrophoresis, which achieves analyte separation and focusing with only one spatially varying force, an electric field gradient. A model for the method is developed and used to analyze peak capacity. Experimental results for a protein ( R ‐phycoerythrin) are given and compared to the model.