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Intracellular FITC‐derivatization with PEG
Author(s) -
Chen Fanguo,
Zhi Daying,
Xia Guangmin
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410300
Subject(s) - derivatization , intracellular , lysis , peg ratio , chemistry , chromatography , protoplast , amino acid , biochemistry , high performance liquid chromatography , finance , economics
In order to investigate the amino acids (AAs) in plant cells, we explore an avenue for intracellular derivatization with FITC. In this method, FITC was used to mark AAs in living protoplasts derived from embryogenic calli of common wheat ( Triticum aestivum L. c.v. Jinan 177) mediated by PEG. After FITC‐derivatization, the AAs in the lysate were determined by CE. The result reveals that this PEG method can be used to transfer FITC into plant cells efficiently, which provides a good method for AA analysis in plant cells.