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Cyclical electrical field flow fractionation
Author(s) -
Gale Bruce K.,
Srinivas Merugu
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410296
Subject(s) - fractionation , field flow fractionation , polystyrene , current (fluid) , range (aeronautics) , field (mathematics) , flow (mathematics) , electric field , materials science , voltage , analytical chemistry (journal) , mechanics , chemistry , chromatography , electrical engineering , physics , thermodynamics , engineering , mathematics , polymer , composite material , quantum mechanics , pure mathematics
Cyclical electrical field flow fractionation (Cy/ElFFF) is demonstrated in a standard electrical field flow fractionation (ElFFF) channel for the first time. Motivation for the use of alternating current (AC) fields in a traditionally direct current (DC) technique are discussed. The function of the system over a wide range of operating conditions is explored and challenges associated with various operating conditions reported. Retention of polystyrene nanoparticle standards is accomplished and the effect of varying parameters of the applied field, such as voltage and frequency, are explored. The first separations using this technique are demonstrated. The experimental results are compared to analytical models previously reported in the literature. The general trend of the experimental results is similar to those predicted in theoretical models and possible reasons for discrepancies are elucidated. Suggestions are made for improving the separation and analysis method, and possible applications explored.