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Portable capillary electrophoresis system with potential gradient detection for separation of DNA fragments
Author(s) -
Xu Yan,
Qin Weidong,
Li Sam F. Y.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410293
Subject(s) - capillary electrophoresis , miniaturization , chromatography , detection limit , electrophoresis , chemistry , dna , analytical chemistry (journal) , capillary action , nanotechnology , materials science , biochemistry , composite material
A portable capillary electrophoresis (CE) system with a novel potential gradient detection (PGD) was utilized to separate DNA fragments. For the first time it was demonstrated that separation of DNA fragments in polymer solution could be detected by a portable CE system integrated with PGD, with a limit of detection (LOD) comparable to that of the CE‐ultraviolet (UV) method. Effects of buffer solution, sieving medium, and applied voltage were also investigated. The portable CE‐PGD system shows several potential advantages, such as simplicity, cost effectiveness, and miniaturization.