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Lipid vesicles in capillary electrophoretic techniques: Characterization of structural properties and associated membrane‐molecule interactions
Author(s) -
Owen Rebecca L.,
Strasters Joost K.,
Breyer Emelita D.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410288
Subject(s) - vesicle , characterization (materials science) , chemistry , capillary electrophoresis , electrophoresis , membrane , molecule , chromatography , capillary action , lipid vesicle , biophysics , materials science , nanotechnology , biochemistry , biology , organic chemistry , composite material
This paper reviews the use of lipid vesicles as model membranes in capillary electrophoresis (CE). The history and utility of CE in the characterization of microparticles is summarized, focusing on the application of colloidal electromigration theories to lipid vesicles. For instance, CE experiments have been used to characterize the size, surface properties, enclosed volumes, and electrophoretic mobilities of lipid vesicles and of lipoprotein particles. Several techniques involving small molecules or macromolecules separated in the presence of lipid vesicles are discussed. Interactions between the analytes and the lipid vesicles – acting as a pseudostationary phase or coated stationary phase in electrokinetic chromatography (EKC) – can be used to obtain additional information on the characteristics of the vesicles and analytes, and to study the biophysical properties of membrane‐molecule interactions in lipid vesicles and lipoproteins. Different methods of determining binding constants by EKC are reviewed, along with the relevant binding constant calculations and a discussion of the application and limitations of these techniques as they apply to lipid vesicle systems.