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Rapid separation of protein isoforms by capillary zone electrophoresis with new dynamic coatings
Author(s) -
Chang William W. P.,
Hobson Christine,
Bomberger David C.,
Schneider Luke V.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410283
Subject(s) - gene isoform , isoelectric focusing , transferrin , capillary electrophoresis , isoelectric point , chemistry , chromatography , electrophoresis , biochemistry , enzyme , gene
Many cellular functions are regulated through protein isoforms. Changes in the expression level or regulatory dysfunctions of isoforms often lead to developmental or pathological disorders. Isoforms are traditionally analyzed using techniques such as gel‐ or capillary‐based isoelectric focusing. However, with proper electroosmotic flow (EOF) control, isoforms with small p I differences can also be analyzed using capillary zone electrophoresis (CZE). Here we demonstrate the ability to quickly resolve isoforms of three model proteins (bovine serum albumin, transferrin, α 1 ‐antitrypsin) in capillaries coated with novel dynamic coatings. The coatings allow reproducible EOF modulation in the cathodal direction to a level of 10 ‐9 m 2 V ‐1 s ‐1 . They also appear to inhibit protein adsorption to the capillary wall, making the isoform separations highly reproducible both in peak areas and apparent mobility. Isoforms of transferrin and α 1 ‐antitrypsin have been implicated in several human diseases. By coupling the CZE isoform separation with standard affinity capture assays, it may be possible to develop a cost‐effective analytical platform for clinical diagnostics.