Determination of dissociation constants of flavonoids by capillary electrophoresis

Ionization constants of some flavanols (catechin and epicatechin) and flavonols (kaempherol, fisetin, morin, and quercetin) are determined by capillary zone electrophoresis (CZE). This technique allows the determination of p K a values until about 12. The p K a values obtained are compared with those calculated by the SPARC computational program. This program predicts the microscopic and macroscopic p K a values and the order of deprotonation of the different −OH groups. While for catechin and epicatechin the first ionizable OH group occurs in ring 1 and the second ionizable group in ring 2, in flavonols the first deprotonation occurs in ring 2 and the second in ring 1.