Premium
An ultrasensitive method for the determination of thiouracil and phenylthiouracil using capillary zone electrophoresis and laser‐induced fluorescence detection
Author(s) -
PérezRuiz Tomás,
MartínezLozano Carmen,
Sanz Antonio,
Galera Raquel
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410252
Subject(s) - capillary electrophoresis , chromatography , laser induced fluorescence , analyte , derivatization , detection limit , fluorescence , chemistry , capillary action , electrophoresis , analytical chemistry (journal) , mass spectrometry , materials science , physics , composite material , quantum mechanics
This paper reports the development of a method based on capillary electrophoresis with laser‐induced fluorescence detection for the simultaneous determination of thiouracil (TU) and phenylthiouracil (PhTU) with high sensitivity (nanomolar range, i.e., attomoles detected). After derivatization with 5‐iodoacetamidofluorescein, the analytes were separated by capillary zone electrophoresis using 20 m M phosphate buffer (pH 10.0) and quantified by fluorescence detection. The linearity range, precision, recovery, and detection limits were determined, and the method was shown to be applicable for the determination of TU and PhTU in spiked feed samples and urine.