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Enantiomeric separation by capillary electrochromatography using monolithic capillaries with sol‐gel‐glued cyclodextrin‐modified silica particles
Author(s) -
Wistuba Dorothee,
Banspach Leena,
Schurig Volker
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410251
Subject(s) - capillary electrochromatography , monolith , monolithic hplc column , electrochromatography , chromatography , cyclodextrin , capillary action , enantiomer , elution , chemistry , chiral column chromatography , theoretical plate , resolution (logic) , capillary electrophoresis , analytical chemistry (journal) , materials science , high performance liquid chromatography , composite material , organic chemistry , artificial intelligence , computer science , catalysis , biochemistry
By an on‐column sol‐gel process, a chiral monolithic stationary phase was prepared by the fusion of permethyl‐β‐cyclodextrin‐silica (Chira‐Dex‐silica) particles and by linking them to the internal capillary wall. The resulting monolith is stable toward voltage (30 kV) and pressure (300 bar) and possesses a high efficiency (up to 100 000 theoretical plates per meter). Efficient enantiomeric separation of various chiral compounds by pressure‐supported capillary electrochromatography (CEC) was achieved. When comparing this method to capillary liquid chromatography (LC) employing the same column in an unified equipment, CEC shows a twofold higher column efficiency at comparable elution times and hence better resolution factors.