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Electrochemiluminescence quenching as an indirect method for detection of dopamine and epinephrine with capillary electrophoresis
Author(s) -
Kang Jianzhen,
Yin XueBo,
Yang Xiurong,
Wang Erkang
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410247
Subject(s) - capillary electrophoresis , electrochemiluminescence , detection limit , epinephrine , chemistry , chromatography , analyte , dopamine , phosphate buffered saline , analytical chemistry (journal) , electrophoresis , medicine
An electrochemiluminescence (ECL) inhibition method was developed as an indirect detection method for the determination of dopamine and epinephrine separated by capillary electrophoresis (CE). When the concentration of Ru(bpy) 3 2+ was 50 μ M diluted by 50 m M phosphate (pH 8.5) in the cell and 0.5 M tripropylamine (TPA) was added to the running buffer (10 m M phosphate, pH 9.0), an inhibition of ECL of the Ru(bpy) 3 2+ /TPA system by the analytes was observed. Under the optimized conditions, the relative standard deviations of migration time and negative peak area were less than 1% and 3%, respectively, for 1 μ M dopamine or 1 μ M epinephrine ( n = 10). Linear ranges of 0.1–10 μ M for both analytes and the detection limits (signal‐to‐noise ratio S/N = 3) of 10 n M for dopamine and 30 n M for epinephrine were obtained.