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Analysis of lamotrigine and its metabolites in human plasma and urine by micellar electrokinetic capillary chromatography
Author(s) -
Pucci Vincenzo,
Bugamelli Francesca,
Baccini Cesare,
Raggi Maria Augusta
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410208
Subject(s) - micellar electrokinetic chromatography , chromatography , urine , chemistry , lamotrigine , capillary electrophoresis , capillary action , medicine , materials science , biochemistry , epilepsy , composite material , psychiatry
A reliable micellar electrokinetic capillary chromatographic method was developed and validated for the determination of lamotrigine and its metabolites in human plasma and urine. The variation of different parameters, such as pH of the background electrolyte (BGE) and Sodium dodecyl sulfate (SDS) concentration, were evaluated in order to find optimal conditions. Best separation of the analytes was achieved using a BGE composed of 10 m M borate and 50 m M SDS, pH 9.5; melatonin was selected as the internal standard. Isolation of lamotrigine and its metabolites from plasma and urine was accomplished with an original solid‐phase extraction procedure using hydrophilic‐lypophilic balance cartridges. Good absolute recovery data and satisfactory precision values were obtained. The calibration plots for lamotrigine and its metabolites were linear over the 1–20 μg/mL concentration range. Sensitivity was satisfactory; the limits of detection and quantitation of lamotrigine were 500 ng/mL and 1 μg/mL, respectively. The application of the method to real plasma samples from epileptic patients under therapy with lamotrigine gave good results in terms of accuracy and selectivity, and in agreement with those obtained with an high‐performance liquid chromatography (HPLC) method.*