Premium
Precision improvement for the analysis of flavonoids in selected Thai plants by capillary zone electrophoresis
Author(s) -
Suntornsuk Leena,
Anurukvorakun Oraphan
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410203
Subject(s) - rutin , chemistry , chromatography , capillary electrophoresis , kaempferol , chromolaena odorata , quercetin , myricetin , centella , detection limit , flavonoid , biochemistry , botany , weed , biology , antioxidant
A capillary zone electrophoresis (CZE) method for the analyses of kaempferol in Centella asiatica and Rosa hybrids and rutin in Chromolaena odorata was developed. The optimization was performed on analyses of flavonoids ( e.g. , rutin, kaempferol, quercetin, myricetin, and apigenin) and organic carboxylic acids ( e.g. , ethacrynic acid and xanthene‐9‐carboxylic acid) by investigation of the effects of types and amounts of organic modifiers, background electrolyte concentrations, temperature, and voltage. Baseline separation ( R s = 2.83) of the compounds was achieved within 10 min in 20 m M NaH 2 PO 4 – Na 2 HPO 4 (pH 8.0) containing 10% v/v ACN and 6% v/v MeOH using a voltage of 25 kV, a temperature of 30°C, and a detection wavelength set at 220 nm. The application of the corrected migration time ( t c ), using ethacrynic acid as the single marker, was efficient to improve the precision of flavonoid identification (% relative standard deviation (RSD) = 0.65%). The method linearity was excellent ( r 2 > 0.999) over 50–150 µg/mL. Precision (%RSD < 1.66%) and recoveries were good (> 96% and %RSDs < 1.70%) with detection and quantitation limits of 2.23 and 7.14 µg/mL, respectively. Kaempferol in C. asiatica and R. hybrids was 0.014 g/100 g (%RSD = 0.59%) and 0.044 g/100 g (%RSD = 1.04%), respectively, and rutin in C. odorata was 0.088 g/100 g (%RSD = 0.06%).