Premium
Separation strategy for acidic chiral pharmaceuticals with capillary electrochromatography on polysaccharide stationary phases
Author(s) -
Mangelings Debby,
Tanret Indiana,
Matthijs Nele,
Maftouh Mohamed,
Massart D. Luc,
Vander Heyden Yvan
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410190
Subject(s) - capillary electrochromatography , electrochromatography , ammonium formate , electrolyte , chemistry , acetonitrile , resolution (logic) , chromatography , ammonium , phase (matter) , formate , stationary phase , organic chemistry , electrode , computer science , catalysis , artificial intelligence
The effect of five factors on the capillary electrochromatographic enantioseparation of acidic compounds was studied using an experimental design. The studied factors were pH, acetonitrile content in the mobile phase, temperature, buffer concentration, and applied voltage. These experiments allowed defining a generic separation strategy applicable on acidic compounds with chemical and structural diversity. The starting screening conditions consist of a 45 m M ammonium formate electrolyte at pH 2.9 mixed with 65% acetonitrile, an applied voltage of 15 kV, and a temperature of 25°C. The screening phase occasionally can be followed by an optimization procedure. Evaluation of the proposed strategy pointed out that it allows achieving baseline resolution within a relatively short time when a beginning of separation is obtained at the starting conditions. This strategy revealed enantioselectivity for 11 compounds out of 15, of which 10 could be baseline‐separated after the proposed optimization steps.