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Determination of ceftazidime in plasma and cerebrospinal fluid by micellar electrokinetic chromatography with direct sample injection
Author(s) -
Yeh HsinHua,
Yang YuanHan,
Chou YuWei,
Ko JuYun,
Chou ChiaAn,
Chen SuHwei
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410185
Subject(s) - chromatography , micellar electrokinetic chromatography , chemistry , ceftazidime , detection limit , sodium dodecyl sulfate , electrokinetic phenomena , biology , bacteria , pseudomonas aeruginosa , genetics
A simple micellar electrokinetic chromatography (MEKC) with UV detection at 254 nm for analysis of ceftazidime in plasma and in cerebrospinal fluid (CSF) by direct injection without any sample pretreatment is described. The separation of ceftazidime from biological matrix was performed at 25°C using a background electrolyte consisting of Tris buffer with sodium dodecyl sulfate (SDS) as the electrolyte solution. Under optimal MEKC condition, good separation with high efficiency and short analyses time is achieved. Several parameters affecting the separation of the drug from biological matrix were studied, including pH and concentration of the Tris buffer and SDS. Using cefazolin as an internal standard (IS), the linear ranges of the method for the determination of ceftazidime in plasma and in CSF were all over the range of 3–90 μg/mL; the detection limit of the drug in plasma and in CSF (signal‐to‐noise ratio = 3; injection 0.5 psi, 5 s) was 2.0 μg/mL. The applicability of the proposed method for determination of ceftazidime in plasma and CSF collected after intravenous administration of 2 g ceftazidime in patients with meningitis was demonstrated.