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Electrokinetic properties of noncharged lipid nanocapsules: Influence of the dipolar distribution at the interface
Author(s) -
Vonarbourg Arnaud,
Saulnier Patrick,
Passirani Catherine,
Benoit JeanPierre
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410145
Subject(s) - electrokinetic phenomena , isoelectric point , ethylene glycol , nanocapsules , electrophoresis , chemical engineering , chemistry , chromatography , peg ratio , lecithin , surface charge , polyelectrolyte , nanoparticle , organic chemistry , polymer , finance , engineering , economics , enzyme
Lipid nanocapsules (LNCs) containing poly(ethylene glycol) (PEG) were developed according to a phase inversion process without organic solvent. The distribution of PEG chains at the surface was determined due to electrokinetic properties, in order to correlate it with protein adsorption potentiality. In this aim, electrophoretic mobilities were measured as a function of ionic strength and pH, for particles differing by their size, dialysis effects, and the presence or not of lecithin in their shell. The study allowed the determination of the isoelectric point (p I ) as well as the charge density ( ZN ) in relation with the dipolar distribution in the polyelectrolyte accessible layer (depth = 1/λ), by using soft‐particle electrophoresis analysis. These parameters pointed out that the PEG surface organization was dependent on the particle size. Moreover, this organization could be modified by dialyzing particles and/or by formulating them with or without lecithin. Lecithin was found to be present in the inner part of the polyelectrolyte layer and to play a role in the outer part disorganization. Dialyzing LNCs formulated with lecithin allowed to obtain stable and well‐structured nanocapsules, ready to an in vivo use as drug delivery system.